The unexpected detection of potato moptop viruspmtv in potatoes growing at several locations in the u. Detection of potato mop top virus and tobacco rattle virus using a multiplex realtime fluorescent reversetranscription polymerase chain reaction assay. Tswv is transmitted by thrips and has the largest host range of any plant virus infecting more than a thousand plant species from 84 families parella et al. Potato moptop virus pmtv causes spraing, necrotic arcs or flecks in the flesh of tubers. Pmtv is one of the causes of spraing disease, the other being tobacco rattle virus see below. Pvy may be transmitted to potato plants through grafting, plant sap inoculation and through aphid transmission. Potato moptop virus is seed and soilborne, and vectored by spongospora subterranea f. Once established in a field, pmtv has the potential of persisting for many years, mainly.
The virus was first identified in 1966 by calvert and harrison in britain, and is now reported in many other potato cultivating regions of the world. Gudmestad, department of plant pathology, north dakota state university, fargo 58108 abstract domfeh, o. It is an important disease in the potato growing areas of the world. Use this page to stay up to date with the response to pmtv. Viewed 6206 times potato mop top virus pmtv, which appears to be increasing in importance in the united states, is a soilborne virus. Effect of soil moisture management on the development of. Virus description and symptoms potato mop top virus pmtv is a pomovirus, and is transmitted by the pathogen a protozoan with zoospores for infection which causes powdery scab disease, spongospora subterranea. Pdf potato moptop virus pmtv is a plant pathogenic virus that affects potatoes.
It is not a food safety issue and people can trust potatoes to eat. We do not use these to store personal information about you. Evaluation of losses caused in the potato variety ica purace by potato virus x, potato virus y and potato leaf roll virus. Rotylenchulus reni ormis ditylenchus dipsaci ditylenchus destructor nacobbus aberrans meloidovne chitwoodi meloidogvnefa. This is a significant cause of rejections of both ware and processing crops. Complete genomic characterization of a potato moptop virus isolate from the united states. Potato moptop virus pmtv00overview eppo global database. Potato moptop virus pmtv is a plant pathogenic virus that affects potatoes. Here we report the complete sequence of the coat protein cp encoding rna of an isolate of pmtv obtained from the field in sweden. Pdf potato moptop virus pmtv is a member of the genus pomovirus and has a singlestranded.
Rtpcr results showed that the symptomatic tuber produced a band at 416 bp with the pmtv primers, which was also present in the pmtvpositive control. Potato moptop virus pmtv is the type member of the genus pomovirus. Our catalog 2020 is now available as download as pdf file. Reaction of potato varieties to potato moptop virus. There are some 40 viruses infecting cultivated potatoes in the field. Molecular data on potato moptop virus pmtv, genus pomovirus, is currently mostly based on analysis of two scottish isolates, pmtvs and pmtvt. The virus was initially reported from germany but now it has spread throughout europe, asia, south america and north. It is confined to areas with a cool and humid climate and is found in northern europe, northern america, china, japan and the andes region. Nicolaisen, danish institute of agricultural sciences, research center flakkebjerg, dk4200 slagelse powdery scab and moptop virus research in denmark has previously, and continues to be focused on pmtv. Pmtv in the united states was first reported in maine 2. Detection of potato viruses x and y in sap extracts by a modified indirect enzymelinked. This virus is spread by the fungus spongospora subterranea f.
Our data show that this rna 34 nt is the second largest of the three rna species in the tripartite pmtv. Potato moptop virus pmtv, the type species of the genus pomovirus, is transmitted by zoospores of the soilborne fungus spongospora subterranean f. Pmtv was first reported in britain in 1966 and subsequently discovered in cooler areas of europe, in the andes of south america, and in asia. Potato leafroll virus an overview sciencedirect topics. Potato moptop virus potato moptop virus pmtv is a viral pathogen of potatoes that affects tuber quality. Pomovirus is a genus of viruses, in the family virgaviridae. Detection of potato mop top virus furovirus on potato in. If you desire to recieve a personal copy with price information. Our catalog 2020 can now be downloaded as pdf file. The unexpected detection of potato moptop virus pmtv in potatoes growing at several locations in the u. Distribution and diversity of potato moptop virus in sweden. The potato moptop virus causes tuber quality problems. Ramesh sv1, raikhy g, brown cr, whitworth jl, pappu hr. Tobacco rattle virus trv and potato mop top virus pmtv are important diseases of potato that are difficult to diagnose reliably by visual symptoms.
Pmtv is in the viral genus pomovirus and is a furovirus fungustransmitted, rodshaped virus. If you wish to recieve a full catalog or further product information, please send us an. Pmtv is one of two viruses causing spraing in potato. The importance of potato mop top virus pmtv in scottish seed potatoes ref. Similar to other plant viruses, pmtv needs a vector to be transmitted from plant to plant. Virus diseases and mycoplasmas 44 calico 45 corky ring spot 45 haywire green dwarf disease 46 interveinal mosaic supermild mosaic or paracrinkle 46 latent mosaic potato virus x, potato mottle virus 47 leafroll 49 mild mosaic 50 purpletop wilt and related diseases 51 rugose mosaic potato virus y 52 spindle tuber 53.
Potato mop top virus is seed and soilborne, and vectored by spongospora subterranea f. Another virus that is able to infect tubers is potato moptop pomovirus pmtv, which is known in other parts of the world as the major reason for spraing in potato. Beet soilborne virus beet virus q broad bean necrosis virus colombian potato soilborne virus potato moptop virus. Potato mop top virus and its growing importance in the u. Infection on tubers may be expressed as arcs or rings on the tuber surface, deep cracking and distortions to the skin that compromising tuber quality.
Additionally, avoiding pmtv or powdery scabinfected seed tubers and using cultivars that. Potato moptop virus is of economic importance to potato growers throughout the. As of spring 2009, the known north american distribution of. Also causing internal necrosis, potato mop top virus pmtv, vectored by powdery scab spongospora. Powdery scab is very widely distributed but pmtv has a more narrow distribution. Potato mop top virus pmtv sutton bridge csr back to gallery item 19 of 26 previous next description. All potato tubers were negative for potato virus y and potato leaf roll virus in replicated elisas agdia, elkhart, in. The behaviour of potato moptop virus in soil, and evidence for its transmission by spongospora subterranea wallr. In the 2006 cara study, planting pmtvfree seed in pmtv infested soil. This virus differs from the other major potato viruses in that it is transmitted by the. Potato, viruses, and seed certification in the usa to. In this study, a new sybr green qpcr qrtpcr and a nested rtpcr nrtpcr were developed to detect potato moptop virus pmtv in potato tuber tissues. Total rna extracted from these tubers were tested by rtpcr at aberdeen, idaho, with primers specific for potato mop top virus pmtv 1 and tobacco rattle virus trv 4.
The most common manner of pvy infection of plant material in the field is through the aphid, and although aphids on their own can directly damage potato plants, it is their role as viral vectors which has the greatest economic impact. Save map as png save map as svg save list as excel file save list as csv file. Reaction of potato varieties topotato moptop virus infection in the. Care must be taken not to infest fields with pmtv from known powdery scab and pmtv infected fields.
Most are poisonous, but many bear edible fruits, leaves, or tubers, and the genus includes several cultivated species, including three major food crops. Moptop virus, relationship to its vector springerlink. Continuing to use this website means you agree to our use of cookies. Also, the two viruses have different effects on chenopodium amaranticolor, and potato. Infections are commonly associated with tuber internal blemishing see also trv. Wisconsin department of agriculture, trade and consumer. Complete genomic characterization of a potato moptop.
The importance of potato mop top virus pmtv in scottish. Once established in fields, powdery scab can survive for up to 18 years in the absence of a potato crop. Besides being a pathogen, powdery scab spongospora subterranea wallr. Effect of soil moisture management on the development of potato moptop virusinduced tuber necrosis o. The most important viruses in north america include potato leafroll virus, potato virus y, x, a, s, m, tobacco rattle virus, and potato mop top virus. The virus is spread from field to field in viruscontaining resting spores carried by potato tubers or in contaminated soil. The disease, potato mop top, is caused by the virus, potato moptop virus pmtv.
Evolutionary divergence of potato moptop virus pmtv, a tripartite singlestranded rna virus, is exceptionally low, based on the analysis of sequences obtained from isolates from europe, asia. Detection of potato mop top virus and tobacco rattle virus. Past and current research on powdery scab and potato mop. Past and current research on powdery scab and potato moptop virus s. Pmtv is an important pathogen of potato, causing significant economic losses in northern europe, north and south america, and asia 3. Potato moptop virus pmtv is a plant pathogenic virus transmitted through the vector spongospora subterranea that affects potatoes. Possible validation of rt pcr n and c europe peru affect tuber quality fungi potato yellow vein virus pyvv order. Symptoms induced by potato moptop virus in potato resemble those caused by tobacco rattle virus, although most potato cultivars react somewhat differently to the two viruses. Chemical treatments for field application against viruses are currently not. Potato moptop virus potato spindle tuber viroid potato virus t potato yellow dwarf virus potato yellow vein virus tobacco rattle virus tobacco ringspot virus stolbur h o lasma globodcra spp. Introduction sss has a narrower host range, infecting members of families potato moptop virus pmtv belongs to family virgaviridaae and solanaceae. Pmtv can induce necrotic arcs or lines in tuber flesh, called spraing disease, in susceptible potato cultivars, rendering the symptomatic tubers unmarketable and.
Pdf first report of potato moptop virus infecting potatoes in alberta. The readthrough region of potato moptop virus pmtv coat. Potato moptop virus an overview sciencedirect topics. Industry research articles idaho potato commission. Spraing is the term given to a range of internal and external symptoms in potato tuber flesh consisting of brown, necrotic rings, arcs and flecks. The virus is transmitted by the soilborne fungus which. The polerovirus potato leafroll virus plrv, the potyviruses pvy and pva fig. Potato mop top virus pmtv sutton bridge csr back to gallery item 19 of 26 previous next. Because the virus is not passed to all the progeny tubers of an infected potato plant, it is gradually selfeliminating in potato stocks grown on virusfree soil. Pmtv belongs to family of virgaviridae, and is the type member of the genus pomovirus potato moptop virus. Andy robinson, potato extension agronomist, ndsuuniversity of minnesota. Potato moptop virus pmtv00documents eppo global database. Documents about potato mop top virus pmtv00 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Solanum is a large, variable genus of annual plants and perennial plants, forbs, vines, subshrubs, shrubs, and small trees.
Development of sybr green realtime pcr and nested rtpcr. Potato moptop virus an emerging problem in the columbia basin jim crosslin, launa hamlin, rich quick, and chuck brown usdaars, prosser, wa a potato pathogen that is increasing in importance in the columbia basin is potato moptop virus pmtv. Two viruses can cause these symptoms, potato moptop virus pmtv and tobacco rattle virus trv. Subterranean, which causes potato powdery scab disease 1,2. Published in the march 2014 issue published online. The methods for chemical control of virus disease are. Potato mop top virus is a plant virus that largely affects potatoes and its a notifiable and unwanted organism in new zealand under the biosecurity act. There are currently four species in this genus including the type species potato moptop virus. The vector of pmtv is the organism that causes another potato disease called powdery scab. Pomovirus y elisa polyclonal bioreba y ncm elisa on host range plants exhibiting symptoms. Effect of soil moisture management on the development ofpotato moptop virusinduced tuber.
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